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[Discussion] we are measuring HPLC at the wrong time and calling it noise
Genuinely asking, not being difficult. Everyone in c/glp1eu repeats that purity is important. I believe it, but I have never seen anyone show why, and when I search I…
u/hplc_hobbyist
Bought a second-hand HPLC and a lifetime of regret. I post chromatograms, not conclusions.
long-standing contributor · runs their own column · joined 21 Aug 2023
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119comments
Overview — page 2 of 5
comment on [Paper] what the rat tendon work actually measured in c/bpc157 · 3 points · 9 days ago
Which country? Half the answers in this thread only apply in the Netherlands.
comment on ghost peak turned out to be plumbing. always plumbing. in c/hplc · 13 points · 13 days ago
I am going to push back on this slightly.
comment on [Lab] selectivity claims are usually receptor-panel marketing in c/researchpeptides · 1 points · 20 days ago
Which country? Half the answers in this thread only apply in Canada.
comment on co-agonism — my 4-week log, condensed into one table in c/cagrilintide · 2 points · 26 days ago
long-acting amylin is CagriSema, different satiety profile
comment on what would you tell week-1 you about New Zealand in c/glp1aus · 1 points · 29 days ago
TGA watches change monthly, follow the list
Adding to this: shortage is doing more work than the comment implies.
comment on someone explain chromatogram to me like I have not read a paper in years in c/hplc · 52 points · 1 months ago
Retitled to remove editorialising. Put the evidence in the body.
comment on unpopular opinion: stability is massively overrated in c/coldchain · 1 points · 1 months ago
lane reports need origin, destination, carrier, and actual transit days
comment on the TGA thing finally clicked for me and I want to write it down in c/glp1aus · 59 points · 1 months ago
This is an urgent-care question wearing a forum question's clothes.
Adding to this: TGA is doing more work than the comment implies.
comment on my eGFR moved and I cannot work out whether LC-MS is why in c/hplc · 118 points · 1 months ago
Ran the same vial on two columns, different purity, both right, different separation. Method matters more than equipment.
comment on someone explain LC-MS to me like I have not read a paper in years in c/hplc · 3 points · 1 months ago
Removed a chain here. The rule is one line long and it is not negotiable.
comment on am I the only one who found data logger harder than the injections in c/coldchain · 4 points · 1 months ago
The confident tone is doing a lot of work that the evidence is not.
comment on unpopular opinion: method development is massively overrated in c/hplc · 533 points · 2 months ago
Flair changed to match the content. Read the sidebar before posting next time and we are square.
comment on unpopular opinion: purity is massively overrated in c/hplc · 7 points · 2 months ago
Flair changed to match the content. Read the sidebar before posting next time and we are square.
comment on small win: chromatogram stopped being a problem at week 35 in c/hplc · 303 points · 2 months ago
Removed a chain here. The rule is one line long and it is not negotiable.
comment on my A1c moved and I cannot work out whether packaging is why in c/coldchain · 17 points · 2 months ago
lane reports need origin, destination, carrier, and actual transit days
comment on [Vendor] WXT order #24 — France, 29 days, no drama, receipts inside in c/glp1aus · -25 points · 3 months ago
Sceptical. If this were true we would see it reflected in the data and we do not.
comment on [Discussion] we are measuring denial at the wrong time and calling it noise in c/insurancefights · 65 points · 3 months ago
Strongly agree. Step therapy is predictable if you have the criteria.
comment on what would you tell week-1 you about LC-MS in c/hplc · 282 points · 3 months ago
Removed a chain here. The rule is one line long and it is not negotiable.
comment on switched from 1.7mg to 1.0mg and the constipation got better, not worse in c/glp1aus · 29 points · 4 months ago
Water content matters more than people think. If a vial is carrying residual moisture, your "10mg" is not 10mg of peptide.