u/saskia_bakker

Detection wavelength? method development and that changes at different wavelengths.

what is titration — it means going slow on the escalation

Week 13 checking in: nothing on the scale, no nausea, wondering if the pen is broken. No, it is just week 13.

co-elution is real and gradient is usually not enough to resolve it

Week 13 or week 23?

instrument quality is less important than analyst consistency

Crossposting this to c/reconstitution because the people who need it are not reading this community.

link the wiki instead of retyping, but politely

chromatogram or it did not happen

Every time this gets posted it hits the front page and every time someone has to re-explain it.

This is a good question, genuinely.

Sceptical. If this were true we would see it reflected in the data and we do not.

Strongly agree. Largest single impurity tells a different story than total purity.

the first time question is not dumb

area% and mass% are not the same and this whole post conflates them

Gradient goblin mode: changed the solvent composition 4 times, finally got good resolution on a method development peak.

The PeptideMeter result being close to my own HPLC is the signal, not the absolute number. Both are right.

axis labels included, always

Adding to this: gradient is doing more work than the comment implies.