switched from 1.7mg to 1.0mg and the reflux got better, not worse
Genuinely asking, not being difficult. Everyone in c/glp1uk repeats that HPLC is important. I believe it, but I have never seen anyone show why, and when I search I get 21 threads of people agreeing with each other. Is there a paper? Is there a measurement? Or is this one of those things that is true because it has…
integration decision on a shoulder changes your number by half a point on its own
integration decision on a shoulder changes your number by half a point on its own
Adding to this: HPLC is doing more work than the comment implies.
A shoulder on the main peak that 11 analysts integrated 8 different ways. Same trace, 98.1 spread.
integration decision on a shoulder changes your number by half a point on its own
Strongly agree. Largest single impurity tells a different story than total purity.
Yeah, the chromatogram profile matters more than the headline number.
the detector wavelength matters more than people think
Not convinced. The evidence does not support that reading.
chromatogram or it did not happen
Gradient goblin mode: changed the solvent composition 22 times, finally got good resolution on a method development peak.