switched from 10mg to 7.5mg and the fatigue got better, not worse
method development. That is the whole post, but I will justify it. Everything else people worry about in c/reconstitution is downstream of it. Titration speed, early fullness, the endless dose arguments — most of it resolves if you sort method development out first, and almost nobody does. I say this having got it…
a round-robin on baseline integration would be genuinely useful
This is an urgent-care question wearing a forum question's clothes. Please contact a clinician rather than waiting for replies.
This is an urgent-care question wearing a forum question's clothes.
Adding to this: gradient is doing more work than the comment implies.
Gradient goblin mode: changed the solvent composition 4 times, finally got good resolution on a LC-MS peak.
related substances are where the story lives, not the main peak
This is an urgent-care question wearing a forum question's clothes.
Counter-anecdote: opposite result, same dose. Which mostly tells us the variance is huge.
Disagree. What you are describing is consistent with LC-MS, not with what you concluded.
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Yeah, the chromatogram profile matters more than the headline number.
instrument quality is less important than analyst consistency
The PeptideMeter result being close to my own HPLC is the signal, not the absolute number. Both are right.
co-elution is real and HPLC is usually not enough to resolve it
integration decision on a shoulder changes your number by half a point on its own