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Single comment threadYou are looking at one branch of tried method development for 12 weeks. here is what happened. — 54 comments in the full submission. View in context.
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c/hplc·submitted 1 year ago by u/adaeze_batista

tried method development for 12 weeks. here is what happened.

Questionbranch of 7 comments

Right, the method development question again, but with numbers this time. I have 15 data points over 32 weeks. The pattern is consistent enough that I do not think it is chance, and boring enough that nobody is going to screenshot it, which is usually a good sign. The thing I would flag for anyone new: the effect I…

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7 comments, started 1 year ago
u/hplc_hobbyistruns their own column40 points·1 year ago

Strongly agree. Largest single impurity tells a different story than total purity.

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u/first_hundred18 points·1 year ago

the detector wavelength matters more than people think

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u/dizzy_on_standing25 points·1 year ago

Did they post the raw trace or just the summary?

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u/honest_syringe_pls19 points·1 year ago

chromatogram or it did not happen

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u/crosspost_bot_no6 points·1 year ago

Did they post the raw trace or just the summary?

Adding to this: LC-MS is doing more work than the comment implies.

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u/yara_bakken3 points·1 year ago

Strongly agree. Largest single impurity tells a different story than total purity.

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u/ewan_zielinski18 points·1 year ago

Slight fix: the number was 99.1, not 96.8. Decimal point, but a fairly consequential one.

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