the LC-MS question that gets asked weekly, answered properly
Been lurking in c/t2dglp1 for about 6 months and finally have something worth posting.
Short version: week 18, 2.5mg, and gradient is the bit I still cannot get a straight answer on. I have read the wiki, I have read the last 33 threads, and the answers split roughly down the middle.
What I actually want to know is whether the people saying it does not matter have measured it, or whether it is just repeated confidently. Happy to be told I am overthinking this — I probably am — but I would rather overthink it now than at week 24.
best — the order this archive was captured in
You have restated the marketing copy. What is the actual substance here.
The related substances profile is way more interesting than the main peak %. A 98% with 18 peaks is different from a 98% that is one 13% impurity.
Injection volume and flow rate? Those affect resolution.
instrument quality is less important than analyst consistency
Not to be pedantic but method development and integration are being used interchangeably and they are not interchangeable in real life.
What was the column and what gradient?
This is the "correlation is mechanism" thing again. You changed three variables at once.
Strongly agree. Largest single impurity tells a different story than total purity.
Strongly agree.
Counter-anecdote: opposite result, same dose. Which mostly tells us the variance is huge.
related substances are where the story lives, not the main peak
- 1Injection volume and flow rate? Those affect resolution.5 comments in this branch · started by u/isabela_nilsen