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c/hplc·submitted 1 year ago by u/rania_okonkwo

three years of purity threads, summarised so you do not have to read them

Labbranch of 7 comments

Right, the method development question again, but with numbers this time. I have 11 data points over 24 weeks. The pattern is consistent enough that I do not think it is chance, and boring enough that nobody is going to screenshot it, which is usually a good sign. The thing I would flag for anyone new: the effect I…

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7 comments, started 1 year ago
u/elodie_grimaldi43 points·1 year ago

This is the correct method framing. Column and gradient are load-bearing fields.

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u/rania_okonkwoOP29 points·1 year ago

Edit to your parent would help — the concentration you quoted assumes one scenario and you have written another above it.

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u/samir_falk10 points·1 year ago

the detector wavelength matters more than people think

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u/quiet_reader_9927 points·1 year ago

Detection wavelength? integration and that changes at different wavelengths.

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u/dead_space_doug7 points·1 year ago

chromatogram or it did not happen

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[deleted]5 points·1 year ago

[deleted]

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u/camila_lindqvist2 points·1 year ago

chromatogram or it did not happen

Adding to this: purity is doing more work than the comment implies.

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