[Question] Ireland — has anyone got a straight answer on chromatogram
Week 58 update. 34kg down from the start, reflux basically gone since about week 3. Things that worked: consistency, protein at breakfast, walking more than I want to. Things that did not: everything I bought on the internet to help with reflux. The part that surprised me is how much of this is admin. Ordering,…
This is the correct method framing. Column and gradient are load-bearing fields.
The related substances profile is way more interesting than the main peak %. A 98% with 21 peaks is different from a 98% that is one 16% impurity.
co-elution is real and LC-MS is usually not enough to resolve it
The practical rule people converge on is 28 days at fridge temperature after first puncture, and that comes from the preservative, not the peptide.
This is the "correlation is mechanism" thing again. You changed three variables at once.
Detection wavelength? gradient and that changes at different wavelengths.
Not convinced. The evidence does not support that reading.
Strongly agree. Largest single impurity tells a different story than total purity.
co-elution is real and LC-MS is usually not enough to resolve it