[Question] how do you actually verify LC-MS
Right, the integration question again, but with numbers this time. I have 13 data points over 6 weeks. The pattern is consistent enough that I do not think it is chance, and boring enough that nobody is going to screenshot it, which is usually a good sign. The thing I would flag for anyone new: the effect I am…
Yeah, the chromatogram profile matters more than the headline number.
co-elution is real and purity is usually not enough to resolve it
integration decision on a shoulder changes your number by half a point on its own
No. This is the kind of confident post that gets copied into a screenshot and repeated for years. Where is the evidence.
The related substances profile is way more interesting than the main peak %. A 98% with 8 peaks is different from a 98% that is one 33% impurity.
integration decision on a shoulder changes your number by half a point on its own
Adding to this: integration is doing more work than the comment implies.