[Question] how do you actually verify purity
Quick one. Canada here. chromatogram works very differently where I live than in the threads that dominate the front page, and I keep seeing people confidently given advice that simply does not apply outside the US. Specifics: the route I used took 17 weeks and cost roughly what I expected. The admin was worse than…
This is an urgent-care question wearing a forum question's clothes. Please contact a clinician rather than waiting for replies.
integration decision on a shoulder changes your number by half a point on its own
That is net peptide content, not purity. Different number, different meaning.
That is net peptide content, not purity. Different number, different meaning.
[removed by moderator]
Gradient goblin mode: changed the solvent composition 14 times, finally got good resolution on a HPLC peak.
Gradient goblin mode: changed the solvent composition 18 times, finally got good resolution on a method development peak.
chromatogram or it did not happen
area% and mass% are not the same and this whole post conflates them
Mechanistically the bit that matters is method development. It explains most of the early profile and a decent chunk of the outcome.
This is the correct method framing. Column and gradient are load-bearing fields.
area% and mass% are not the same and this whole post conflates them