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c/hplc·submitted 1 year ago by u/dizzy_on_standing

the gradient question that gets asked weekly, answered properly

Explainerbranch of 6 comments

Genuinely asking, not being difficult. Everyone in c/glp1eu repeats that method development is important. I believe it, but I have never seen anyone show why, and when I search I get 11 threads of people agreeing with each other. Is there a paper? Is there a measurement? Or is this one of those things that is true…

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6 comments, started 1 year ago
u/gustav_solberg36 points·1 year ago

Disagree. What you are describing is consistent with chromatogram, not with what you concluded.

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u/rosa_sandvik27 points·1 year ago

Disagree but this is the good kind of wrong — it is specific enough to be checked.

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u/gustav_solberg13 points·1 year ago

Disagree. What you are describing is consistent with gradient, not with what you concluded.

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u/dizzy_on_standingOP21 points·1 year ago

Gradient goblin mode: changed the solvent composition 7 times, finally got good resolution on a LC-MS peak.

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u/gustav_solberg25 points·1 year ago

I am going to push back on this slightly.

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u/vikram_asante16 points·1 year ago

the gradient is doing the separation work, not the column alone

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