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c/hplc·submitted 6 months ago by u/alcohol_aversion

tried HPLC for 6 weeks. here is what happened.

Correctionbranch of 8 comments

purity. That is the whole post, but I will justify it. Everything else people worry about in c/dosinglogs is downstream of it. Titration speed, muscle cramps, the endless dose arguments — most of it resolves if you sort purity out first, and almost nobody does. I say this having got it wrong for 6 months. My…

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8 comments, started 6 months ago
u/downvote_magnet-4 points·6 months ago

state the method: column, gradient, detection wavelength, injection volume

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u/ewan_zielinski1 point·6 months ago

Strongly agree. Largest single impurity tells a different story than total purity.

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u/alcohol_aversionOP0 points·6 months ago

Detection wavelength? integration and that changes at different wavelengths.

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u/rasmus_petrescu1 point·6 months ago

I am going to push back on this slightly.

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u/rina_nascimento1 point·6 months ago

This is the correct method framing. Column and gradient are load-bearing fields.

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u/ahmed_rasmussen1 point·6 months ago

area% and mass% are not the same and this whole post conflates them

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u/mariam_cabrera1 point·6 months ago

axis labels included, always

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u/honest_syringe_pls1 point·6 months ago

Injection volume and flow rate? Those affect resolution.

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