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c/hplc·submitted 3 months ago by u/rania_okonkwo

what would you tell week-1 you about LC-MS

Labbranch of 4 comments

Right, the purity question again, but with numbers this time. I have 5 data points over 10 weeks. The pattern is consistent enough that I do not think it is chance, and boring enough that nobody is going to screenshot it, which is usually a good sign. The thing I would flag for anyone new: the effect I am describing…

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4 comments, started 3 months ago
u/blunt_coldbox_2024152 points·3 months ago

Water content matters more than people think. If a vial is carrying residual moisture, your "10mg" is not 10mg of peptide.

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u/two_mil_or_one119 points·3 months ago

Water content matters more than people think.

Adding to this: purity is doing more work than the comment implies.

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u/liv_okafor64 points·3 months ago

area% and mass% are not the same and this whole post conflates them

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The analytical deep end: RP-HPLC methods, gradient design, integration decisions, LC-MS confirmation, amino-acid analysis, water content, and why two labs can report 98.1% and 96.4% on the same vial and both be right. Post the chromatogram, not the summary line.

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