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c/coa·submitted 1 year ago by u/kavya_verhoeven

batch vs related substances — genuinely asking which matters more

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Long-ish post, sorry. tl;dr at the bottom. I have been tracking area percent against ApoB for 31 weeks because I could not find anyone who had. The correlation is weaker than I expected, which is itself mildly interesting given how confidently people link the two in here. Caveats up front: one person, one lab, one…

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6 comments, started 1 year ago
u/nikhil_lindqvist101 points·1 year ago

Sent one vial to PeptideMeter and VendorInvestigate. 98.4% and 99.0%. Cost me more than the vial did and I would do it again.

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[deleted]73 points·1 year ago

[deleted]

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u/coa_collectorarchivist33 points·1 year ago

area% is not mass%, and here is exactly why it matters

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u/gallbladder_gary48 points·1 year ago

Batch number and date of analysis? Both are load-bearing.

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u/yara_antonsen20 points·1 year ago

chromatogram or it did not happen

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u/isabela_nilsen7 points·1 year ago

Slight fix: the number was 97.6, not 98.9. Decimal point, but a fairly consequential one.

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How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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