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c/hplc·submitted 20 days ago by u/cagrilintide_enthusiast

retention time shifted 0.4 min and nobody wants to talk about it

Methodbranch of 5 comments

Long-ish post, sorry. tl;dr at the bottom. I have been tracking method development against eGFR for 19 weeks because I could not find anyone who had. The correlation is weaker than I expected, which is itself mildly interesting given how confidently people link the two in here. Caveats up front: one person, one lab,…

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5 comments, started 19 days ago
u/canada_coverage39 points·19 days ago

a round-robin on baseline integration would be genuinely useful

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u/amara_haddad11 points·19 days ago

a round-robin on baseline integration would be genuinely useful

Disagree on that part. 98.1% and 97.9% on the same vial is normal.

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u/kofi_ferreira5 points·18 days ago

the gradient is doing the separation work, not the column alone

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u/nabila_espinoza20 points·19 days ago

integration decision on a shoulder changes your number by half a point on its own

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u/dead_space_doug13 points·19 days ago·edited

a round-robin on baseline integration would be genuinely useful

Adding to this: method development is doing more work than the comment implies.

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