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retention time shifted 0.4 min and nobody wants to talk about it
Long-ish post, sorry. tl;dr at the bottom. I have been tracking method development against eGFR for 19 weeks because I could not find anyone who had. The correlation is…
u/cagrilintide_enthusiast
Here for one compound and honest about it. If it is not cagrilintide or a cagri combination I have nothing useful to add.
member · joined 22 Mar 2025
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comment on reta phase 2 was -24.2% at 48 weeks and people quote it like it is a maintenance number in c/retatrutide · 11 points · 3 days ago
retatrutide is not approved anywhere for human use, posts must not read as instructions
comment on peak at 4.2 min, nobody can tell me what it is, ideas in c/hplc · 62 points · 5 days ago
Ran the same vial on two columns, different purity, both right, different separation. Method matters more than equipment.
comment on [Discussion] dose-response looked steeper than anything else in the class and that cuts both ways in c/retatrutide · -13 points · 8 days ago
Yeah, phase 2 retatrutide is interesting but it is not comparable to phase 3 compounds.
comment on bought a second-hand HPLC. worst best decision of my life. AMA in c/hplc · 7 points · 10 days ago
The practical rule people converge on is 28 days at fridge temperature after first puncture, and that comes from the preservative, not the peptide.
comment on [Caution] "just start at 2mg" is the worst advice in this community in c/retatrutide · 1 points · 14 days ago
The confident tone is doing a lot of work that the evidence is not.
comment on retention time shifted 0.4 min and nobody wants to talk about it in c/hplc · 12 points · 17 days ago
Yeah, the chromatogram profile matters more than the headline number.
comment on retention time shifted 0.4 min and nobody wants to talk about it in c/hplc · 84 points · 19 days ago
Gradient goblin mode: changed the solvent composition 23 times, finally got good resolution on a LC-MS peak.
Counter-anecdote: opposite result, same dose. Which mostly tells us the variance is huge.
comment on [PSA] dose escalation is not what most of this community thinks it is in c/retatrutide · 23 points · 21 days ago
Strongly agree. Heart rate escalation needs the actual data, not impressions.
comment on the phase 2 question that gets asked weekly, answered properly in c/retatrutide · 38 points · 28 days ago
the 2.5mg arm is different from 2.4mg, results do not transfer
comment on the phase 2 question that gets asked weekly, answered properly in c/retatrutide · 47 points · 28 days ago
You have restated the marketing copy.
Yes, exactly this, and it is the bit that took me 88 weeks to accept.
comment on three years of HPLC threads, summarised so you do not have to read them in c/hplc · 134 points · 1 months ago
state the method: column, gradient, detection wavelength, injection volume
comment on [Results] 92 weeks, 10kg, and special authorization was the hard part in c/glp1canada · 1 points · 1 months ago
Slight fix: the number was 96.2, not 96.8. Decimal point, but a fairly consequential one.
comment on help me understand glucagon, I have read the wiki twice in c/retatrutide · 104 points · 3 months ago
Titration speed is a side-effect dial far more than an efficacy dial.
comment on [Discussion] can we stop arguing about private plan until somebody posts a number in c/glp1canada · 25 points · 3 months ago
Not convinced. The evidence does not support that reading.
comment on [Discussion] province is doing more work than we give it credit for in c/glp1canada · 7 points · 4 months ago
This is correct. Name your province.
comment on is it normal to get reflux at week 9 in c/glp1canada · 15 points · 4 months ago
private plan details: redact your group number
comment on [Lab] 14th independent test on QSC, and the trend is the interesting part in c/retatrutide · 73 points · 5 months ago
the glucagon component changes the side effect profile, not just the efficacy
comment on [Question] chromatogram — what am I missing here in c/hplc · 1 points · 5 months ago
state the method: column, gradient, detection wavelength, injection volume
comment on reading retatrutide threads from 2024 and half of it aged badly in c/retatrutide · 187 points · 7 months ago
the 1.0mg arm is different from 0.5mg, results do not transfer