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c/hplc·submitted 2 years ago by u/chain_confirm_c

[Results] 91 weeks on 5mg, full numbers, ask me anything boring

Discussionbranch of 8 comments

Something I noticed reading old threads that I have not seen said out loud. The advice on chromatogram in this community changed substantially around the start of last year, and nobody went back and updated the older posts. So depending on which thread the search engine hands you, you get two contradictory answers…

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8 comments, started 2 years ago
u/hedda_ekstrom159 points·2 years ago·edited

Gradient goblin mode: changed the solvent composition 4 times, finally got good resolution on a chromatogram peak.

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u/anya_erdogan38 points·2 years ago

What was the column and what gradient?

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u/reverse_image_ron56 points·2 years ago

area% and mass% are not the same and this whole post conflates them

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u/fatima_yildiz34 points·2 years ago

Detection wavelength? method development and that changes at different wavelengths.

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u/chain_confirm_cOPpayments15 points·2 years ago

two labs, two different baselines, two different purity numbers, both honest

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u/careful_gradient78 points·2 years ago

This is the correct method framing. Column and gradient are load-bearing fields.

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u/alcohol_aversion112 points·2 years ago

Detection wavelength? integration and that changes at different wavelengths.

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u/marisol_frisk42 points·2 years ago

Disagree. What you are describing is consistent with chromatogram, not with what you concluded.

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