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c/hplc·submitted 4 months ago by u/nnt_nate

8 months in and HPLC is still the thing I get wrong

Methodbranch of 4 comments

Long-ish post, sorry. tl;dr at the bottom. I have been tracking method development against triglycerides for 25 weeks because I could not find anyone who had. The correlation is weaker than I expected, which is itself mildly interesting given how confidently people link the two in here. Caveats up front: one person,…

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4 comments, started 4 months ago
u/hamza_weiss866 points·4 months ago

the gradient is doing the separation work, not the column alone

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u/bilal_adebayo-22 points·4 months ago

the gradient is doing the separation work, not the column alone

Yes, exactly this, and it is the bit that took me 82 weeks to accept.

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u/micro_bump_mick1 point·4 months ago

Strongly agree. Largest single impurity tells a different story than total purity.

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[removed]609 points·4 months ago

[removed by moderator]

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