three years of chromatogram threads, summarised so you do not have to read them
chromatogram. That is the whole post, but I will justify it.
Everything else people worry about in c/dosinglogs is downstream of it. Titration speed, early fullness, the endless dose arguments — most of it resolves if you sort chromatogram out first, and almost nobody does.
I say this having got it wrong for 19 months. My eGFR was the thing that eventually made me pay attention, which is a stupid way to learn a lesson that was in the sidebar the whole time.
best — the order this archive was captured in
Strongly agree. Largest single impurity tells a different story than total purity.
Strongly agree.
Adding to this: HPLC is doing more work than the comment implies.
the detector wavelength matters more than people think
Ran the same vial on two columns, different purity, both right, different separation. Method matters more than equipment.
Strongly agree.
Adding to this: HPLC is doing more work than the comment implies.
area% and mass% are not the same and this whole post conflates them
Strongly agree.
Yes, exactly this, and it is the bit that took me 41 weeks to accept.
What was the column and what gradient?
Strongly agree. Largest single impurity tells a different story than total purity.
Not to be pedantic but LC-MS and chromatogram are being used interchangeably and they are not interchangeable in real life.
area% and mass% are not the same and this whole post conflates them
No. This is the kind of confident post that gets copied into a screenshot and repeated for years. Where is the evidence.
No.
Counter-anecdote: opposite result, same dose. Which mostly tells us the variance is huge.
- 1Strongly agree. Largest single impurity tells a different story than total…8 comments in this branch · started by u/vikram_asante
- 2Strongly agree. Largest single impurity tells a different story than total…5 comments in this branch · started by u/dead_space_doug