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c/hplc·submitted 2 years ago by u/arne_nyberg

[Meta] the method development rule is doing its job and people should stop complaining

Explainerbranch of 5 comments

method development. That is the whole post, but I will justify it. Everything else people worry about in c/dosinglogs is downstream of it. Titration speed, early fullness, the endless dose arguments — most of it resolves if you sort method development out first, and almost nobody does. I say this having got it wrong…

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5 comments, started 2 years ago
u/downvote_magnet524 points·2 years ago
okay but what dose
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u/coa_janitormod · c/coa127 points·2 years ago

axis labels included, always

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u/freya_baptista185 points·2 years ago

The confident tone is doing a lot of work that the evidence is not.

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u/enzo_petrescu145 points·2 years ago

integration decision on a shoulder changes your number by half a point on its own

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The analytical deep end: RP-HPLC methods, gradient design, integration decisions, LC-MS confirmation, amino-acid analysis, water content, and why two labs can report 98.1% and 96.4% on the same vial and both be right. Post the chromatogram, not the summary line.

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