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c/hplc·submitted 1 year ago by u/marit_mwangi

what would you tell week-1 you about gradient

Discussionbranch of 6 comments

Been lurking in c/t2dglp1 for about 11 months and finally have something worth posting. Short version: week 76, 15mg, and method development is the bit I still cannot get a straight answer on. I have read the wiki, I have read the last 15 threads, and the answers split roughly down the middle. What I actually want to…

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6 comments, started 1 year ago
u/arne_nyberg264 points·1 year ago

axis labels included, always

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u/canada_coverage213 points·1 year ago

Strongly agree. Largest single impurity tells a different story than total purity.

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u/arne_nyberg308 points·1 year ago

Strongly agree.

Adding to this: LC-MS is doing more work than the comment implies.

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u/hugo_bergstrom168 points·1 year ago

Not to be pedantic but method development and integration are being used interchangeably and they are not interchangeable in real life.

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u/ferran_krastev363 points·1 year ago

Strongly agree.

Counter-anecdote: opposite result, same dose. Which mostly tells us the variance is huge.

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u/medutest_mel423 points·1 year ago

That is net peptide content, not purity. Different number, different meaning.

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