someone explain LC-MS to me like I have not read a paper in years
Posting this because I searched for it and got nothing useful. Setup: 0.5mg, week 62, constipation present but not ruining anything. I changed exactly one variable — gradient — and tracked it for 15 weeks. Result: a difference, but a small one, and well inside what I would call noise if someone else posted it. So I…
instrument quality is less important than analyst consistency
The related substances profile is way more interesting than the main peak %. A 98% with 13 peaks is different from a 98% that is one 39% impurity.
Disagree but this is the good kind of wrong — it is specific enough to be checked.
Yeah, the chromatogram profile matters more than the headline number.
state the method: column, gradient, detection wavelength, injection volume
Sceptical. If this were true we would see it reflected in the data and we do not.
state the method: column, gradient, detection wavelength, injection volume
instrument quality is less important than analyst consistency