LC-MS: what the trials say vs what this community says
Quick one. Canada here. chromatogram works very differently where I live than in the threads that dominate the front page, and I keep seeing people confidently given advice that simply does not apply outside the US. Specifics: the route I used took 6 weeks and cost roughly what I expected. The admin was worse than…
area% and mass% are not the same and this whole post conflates them
Gradient goblin mode: changed the solvent composition 8 times, finally got good resolution on a LC-MS peak.
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integration decision on a shoulder changes your number by half a point on its own
co-elution is real and chromatogram is usually not enough to resolve it
That is net peptide content, not purity. Different number, different meaning.
Ran the same vial on two columns, different purity, both right, different separation. Method matters more than equipment.
area% and mass% are not the same and this whole post conflates them
Adding to this: LC-MS is doing more work than the comment implies.
state the method: column, gradient, detection wavelength, injection volume