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c/hplc·submitted 3 months ago by u/honest_syringe_pls

[Question] method development — what am I missing here

Questionbranch of 4 comments

Been lurking in c/t2dglp1 for about 3 months and finally have something worth posting. Short version: week 4, 10mg, and HPLC is the bit I still cannot get a straight answer on. I have read the wiki, I have read the last 19 threads, and the answers split roughly down the middle. What I actually want to know is whether…

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4 comments, started 3 months ago
u/enzo_petrescu44 points·3 months ago
i do not know. that is the honest answer.
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u/mass_spec_maggieMOD27 points·3 months ago

Removed a chain here. The rule is one line long and it is not negotiable.

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u/coa_janitormod · c/coa19 points·3 months ago·edited

i do not know.

Disagree on that part. 97.6% and 97.9% on the same vial is normal.

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u/b12_baseline6 points·3 months ago

That is net peptide content, not purity. Different number, different meaning.

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