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c/hplc·submitted 2 months ago by u/rina_bergstrom

what would you tell week-1 you about HPLC

Labbranch of 4 comments

Week 47 update. 43kg down from the start, reflux basically gone since about week 87. Things that worked: consistency, protein at breakfast, walking more than I want to. Things that did not: everything I bought on the internet to help with reflux. The part that surprised me is how much of this is admin. Ordering,…

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4 comments, started 2 months ago
u/careful_gradient102 points·2 months ago

Gradient goblin mode: changed the solvent composition 4 times, finally got good resolution on a LC-MS peak.

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u/vikram_asante36 points·2 months ago

No. This is the kind of confident post that gets copied into a screenshot and repeated for years. Where is the evidence.

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u/peak_area_peteanalytical11 points·2 months ago

axis labels included, always

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u/vikram_asante6 points·2 months ago

Injection volume and flow rate? Those affect resolution.

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