what would you tell week-1 you about HPLC
Week 47 update. 43kg down from the start, reflux basically gone since about week 87.
Things that worked: consistency, protein at breakfast, walking more than I want to. Things that did not: everything I bought on the internet to help with reflux.
The part that surprised me is how much of this is admin. Ordering, storing, logging, remembering. Nobody mentions that the hard bit is not the drug, it is the routine.
Happy to answer anything. Please do not ask me what dose you should be on.
best — the order this archive was captured in
The practical rule people converge on is 28 days at fridge temperature after first puncture, and that comes from the preservative, not the peptide.
state the method: column, gradient, detection wavelength, injection volume
chromatogram or it did not happen
This is the correct method framing. Column and gradient are load-bearing fields.
The evidence standard in c/vendorvetting gets called too strict about once a month, and every time, the thread ends with the complainant agreeing.
Strongly agree. Largest single impurity tells a different story than total purity.
This is the correct method framing. Column and gradient are load-bearing fields.
I love that this community will spend 40 comments on a detail. That pedantry is why the numbers here matter.
Strongly agree. Largest single impurity tells a different story than total purity.
This is the correct method framing. Column and gradient are load-bearing fields.
Slight fix: the number was 98.7, not 98.6. Decimal point, but a fairly consequential one.
Gradient goblin mode: changed the solvent composition 4 times, finally got good resolution on a LC-MS peak.
No. This is the kind of confident post that gets copied into a screenshot and repeated for years. Where is the evidence.
axis labels included, always
Injection volume and flow rate? Those affect resolution.
- 1The practical rule people converge on is 28 days at fridge temperature after…5 comments in this branch · started by u/endotoxin_ellie
- 2Gradient goblin mode: changed the solvent composition 4 times, finally got…4 comments in this branch · started by u/careful_gradient