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c/hplc·submitted 2 months ago by u/rina_bergstrom

what would you tell week-1 you about HPLC

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Week 47 update. 43kg down from the start, reflux basically gone since about week 87. Things that worked: consistency, protein at breakfast, walking more than I want to. Things that did not: everything I bought on the internet to help with reflux. The part that surprised me is how much of this is admin. Ordering,…

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5 comments, started 2 months ago

u/endotoxin_elliemicro761 points·2 months ago

The practical rule people converge on is 28 days at fridge temperature after first puncture, and that comes from the preservative, not the peptide.

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u/nnt_nate264 points·2 months ago

state the method: column, gradient, detection wavelength, injection volume

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[removed]199 points·2 months ago

[removed by moderator]

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About c/hplc

The analytical deep end: RP-HPLC methods, gradient design, integration decisions, LC-MS confirmation, amino-acid analysis, water content, and why two labs can report 98.1% and 96.4% on the same vial and both be right. Post the chromatogram, not the summary line.

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State the method: column, gradient, detection wavelength, injection volume.
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