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c/hplc·submitted 11 days ago by u/micro_bump_mick

bought a second-hand HPLC. worst best decision of my life. AMA

Labbranch of 6 comments

Quick one. Canada here. HPLC works very differently where I live than in the threads that dominate the front page, and I keep seeing people confidently given advice that simply does not apply outside the US. Specifics: the route I used took 2 weeks and cost roughly what I expected. The admin was worse than the cost.…

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6 comments, started 10 days ago
u/cagrilintide_enthusiast7 points·10 days ago

The practical rule people converge on is 28 days at fridge temperature after first puncture, and that comes from the preservative, not the peptide.

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u/micro_bump_mickOP3 points·9 days ago

Titration speed is a side-effect dial far more than an efficacy dial.

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u/micro_bump_mickOP2 points·9 days ago

instrument quality is less important than analyst consistency

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u/aksel_palacios1 point·9 days ago

chromatogram or it did not happen

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u/zeynep_zielinski1 point·9 days ago

Titration speed is a side-effect dial far more than an efficacy dial.

Adding to this: method development is doing more work than the comment implies.

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