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21 months in and HPLC is still the thing I get wrong
Genuinely asking, not being difficult. Everyone in c/glp1uk repeats that chromatogram is important. I believe it, but I have never seen anyone show why, and when I…
u/endotoxin_ellie
LAL testing, sterility, and why "sterile filtered" is a sentence not a certificate.
long-standing contributor · micro · joined 28 Mar 2024
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Overview — page 3 of 5
comment on is it normal to get fatigue at week 90 in c/retatrutide · 582 points · 9 months ago
Not convinced. The evidence does not support that reading.
comment on small win: regulatory stopped being a problem at week 71 in c/bpc157 · 17 points · 10 months ago
Rodent data is rodent data. Dose scaling is not linear and the models tell you what to investigate, not what to expect.
comment on week 76 check-in — 12kg down, fatigue manageable, one thing confusing me in c/labresults · 173 points · 10 months ago
below-spec results get logged, not dismissed
comment on anyone else notice BPC-157 kicking in around week 54 in c/bpc157 · 1 points · 10 months ago
near-zero controlled human data, full stop
comment on 21 months in and HPLC is still the thing I get wrong in c/hplc · 21 points · 11 months ago
the detector wavelength matters more than people think
comment on 21 months in and HPLC is still the thing I get wrong in c/hplc · 41 points · 11 months ago
Gradient goblin mode: changed the solvent composition 17 times, finally got good resolution on a LC-MS peak.
comment on does purity actually matter or is it forum lore at this point in c/hplc · 45 points · 11 months ago
Yeah, the chromatogram profile matters more than the headline number.
comment on [Meta] proposal — a flair for MASH posts in c/survodutide · 20 points · 1 year ago
the MASH indication changes the risk calculus
comment on my ALT moved and I cannot work out whether BPC-157 is why in c/bpc157 · 8 points · 1 year ago
Yeah, rodent data does not scale to humans.
comment on [Results] 61 weeks on 2.4mg, full numbers, ask me anything boring in c/retatrutide · 1 points · 1 year ago
the glucagon component changes the side effect profile, not just the efficacy
comment on [Question] New Zealand — has anyone got a straight answer on insulin resistance in c/glp1women · 35 points · 1 year ago
hair-loss on PCOS is real
comment on 3 months in and chromatogram is still the thing I get wrong in c/hplc · 32 points · 1 year ago
a round-robin on baseline integration would be genuinely useful
comment on phase 2 — my 12-week log, condensed into one table in c/retatrutide · 10 points · 1 year ago
phase 2 numbers are not maintenance numbers, cite the trial
comment on anyone else notice retatrutide kicking in around week 49 in c/retatrutide · 0 points · 1 year ago
The heart rate signal in TRIUMPH is real and concerning.
Disagree on that part. 97.9% and 97.6% on the same vial is normal.
comment on my A1c moved and I cannot work out whether phase 2 is why in c/retatrutide · 99 points · 1 year ago
triple agonism is phase 2, do not generalise
comment on [Vendor] QST order #2 — Denmark, 37 days, no drama, receipts inside in c/bpc157 · 11 points · 1 year ago
the pentadecapeptide literature looks promising but rodent-only
comment on why does nobody talk about dose escalation in c/retatrutide · 11 points · 1 year ago
the glucagon component changes the side effect profile, not just the efficacy
comment on [PSA] test results is not what most of this community thinks it is in c/labresults · 21 points · 1 year ago
Half-life is about 168 hours, so steady state lands around week 23–5. Practical consequence: what you feel in week 1 is not what that dose does.
comment on the Netherlands: shipping, and what it actually costs here in c/intlshipping · 160 points · 1 year ago
This is correct. Variance is the story.