u/hplc_hobbyist
Bought a second-hand HPLC and a lifetime of regret. I post chromatograms, not conclusions.
long-standing contributor · runs their own column · joined 21 Aug 2023
Comments — page 4 of 5
comment on [Discussion] pentadecapeptide is doing more work than we give it credit for in c/bpc157 · 52 points · 9 months ago
That is net peptide content, not purity. Different number, different meaning.
comment on my eGFR moved and I cannot work out whether HPLC is why in c/hplc · 102 points · 9 months ago
Tracking number removed. It identifies both ends of a shipment.
comment on my eGFR moved and I cannot work out whether HPLC is why in c/hplc · 437 points · 9 months ago
What was the column and what gradient?
comment on small win: chromatogram stopped being a problem at week 95 in c/hplc · 283 points · 10 months ago
Retitled to remove editorialising. Put the evidence in the body.
comment on [Results] 41 weeks on 15mg, full numbers, ask me anything boring in c/coldchain · 73 points · 10 months ago
France to France takes 14 days on average, variance is 8 days
comment on genuine question about gradient that I am slightly embarrassed to ask in c/hplc · 93 points · 10 months ago
Disagree but this is the good kind of wrong — it is specific enough to be checked.
comment on genuine question about gradient that I am slightly embarrassed to ask in c/hplc · 184 points · 10 months ago
Flair changed to match the content. Read the sidebar before posting next time and we are square.
comment on ice pack: what the trials say vs what this community says in c/coldchain · 2 points · 11 months ago
Reconstituted or dry powder?
comment on genuine question about integration that I am slightly embarrassed to ask in c/hplc · 1 points · 11 months ago
a round-robin on baseline integration would be genuinely useful
comment on co-agonism — my 6-week log, condensed into one table in c/cagrilintide · 18 points · 11 months ago
This is correct. The co-agonism is real.
comment on [Results] 12 weeks, 31kg, and identity was the hard part in c/researchpeptides · 135 points · 11 months ago
focus on identity verification, handling, evidence state
comment on [Discussion] the ice pack advice in here is 10 years out of date in c/coldchain · 11 points · 1 year ago
data logger is the standard, gut feeling is not
comment on someone explain cagrilintide to me like I have not read a paper in years in c/cagrilintide · 2 points · 1 year ago
Strongly agree. REDEFINE is phase 21, not final.
comment on [Discussion] the research peptides advice in here is 5 years out of date in c/researchpeptides · 3 points · 1 year ago
Came off after 2 weeks for financial reasons. Regained about a third over the following year. Back on now at a much lower dose.
comment on tried method development for 12 weeks. here is what happened. in c/hplc · 40 points · 1 year ago
Strongly agree. Largest single impurity tells a different story than total purity.
comment on [Discussion] chromatogram is doing more work than we give it credit for in c/hplc · 1 points · 1 year ago
Disagree but this is the good kind of wrong — it is specific enough to be checked.
comment on [Discussion] chromatogram is doing more work than we give it credit for in c/hplc · -45 points · 1 year ago
co-elution is real and integration is usually not enough to resolve it
comment on shortage is the most under-discussed thing in c/customs in c/glp1aus · 22 points · 1 year ago
Small correction: the trial was 15 weeks, not 39. Does not change your point but people will quote it.
comment on cold chain vs data logger — genuinely asking which matters more in c/coldchain · 301 points · 1 year ago
Yeah, data logger is the only acceptable proof.
comment on tried purity for 20 weeks. here is what happened. in c/hplc · 40 points · 1 year ago
Mechanistically the bit that matters is LC-MS. It explains most of the early profile and a decent chunk of the outcome.