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c/coa·posted 2 years ago by u/freya_baptista

batch — my 10-week log, condensed into one table

Question Cold Box ×5 Sourced ×3 Long Haul ×3

Posting this because I searched for it and got nothing useful.

Setup: 15mg, week 60, constipation present but not ruining anything. I changed exactly one variable — area percent — and tracked it for 6 weeks.

Result: a difference, but a small one, and well inside what I would call noise if someone else posted it. So I am putting it here rather than presenting it as a finding. If two or three other people have done the same thing we might actually learn something.

Not medical advice, obviously. This is one person with a spreadsheet.

5,321 up / 525 down91% upvoted41 commentsid 1xhtes4 Jul 2024

41 comments

30 in this archive, depth 5

best — the order this archive was captured in

u/receptor_bias_rick0 points·2 years ago
well that is my evening gone, thanks for the paper
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u/sunday_shot_sal367 points·2 years ago

the related substances line is where the story is

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u/freya_baptistaOP169 points·2 years ago

Sent one vial to PeptideMeter and VendorInvestigate. 99.0% and 98.6%. Cost me more than the vial did and I would do it again.

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u/anecdata_annie-38 points·2 years ago

Cosigning. The batch number is load-bearing information.

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u/sunday_shot_sal50 points·2 years ago

That is net peptide content, not purity. Different number, different meaning.

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u/mikkel_correia229 points·2 years ago

This is the correct framing. The COA structure matters as much as the number.

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u/laila_yilmaz58 points·2 years ago

Water content was 3.8%, acetate was 6.2%, purity was 99%, but net peptide was only 90% of the label weight. Perfectly honest, just chemistry.

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u/tidy_vialdrawer_pls190 points·2 years ago·edited

This is the correct framing. The COA structure matters as much as the number.

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u/kavya_verhoeven215 points·2 years ago

Sent one vial to PeptideMeter and VendorInvestigate. 97.9% and 94.2%. Cost me more than the vial did and I would do it again.

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u/cato_achebe103 points·2 years ago

the method line is doing real work, not just window dressing

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u/freya_baptistaOP141 points·2 years ago

The chromatogram trace is where the real information lives. The summary number is just a headline.

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u/halima_boateng66 points·2 years ago

Sent one vial to PeptideMeter and VendorInvestigate.

Disagree on that part. 98.4% and 98.3% on the same vial is normal.

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u/zeynep_zielinski27 points·2 years ago

Small correction: the trial was 2 weeks, not 20. Does not change your point but people will quote it.

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u/priya_palacios19 points·2 years ago

Water content on the document? That affects your concentration math.

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u/freya_baptistaOP18 points·2 years ago

Metadata check caught a COA that was created in a browser four months after the analysis date. Not evidence of fraud, but worth flagging to SGN.

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u/chidi_demir5 points·2 years ago·edited

Metadata check caught a COA that was created in a browser four months after the analysis date.

Yes, exactly this, and it is the bit that took me 8 weeks to accept.

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u/laila_yilmaz60 points·2 years ago

Cosigning. The batch number is load-bearing information.

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u/isabela_nilsen71 points·2 years ago

no "is this good?" without saying what the compound and claimed purity are

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u/kavya_verhoeven44 points·2 years ago

no "is this good?" without saying what the compound and claimed purity are

Adding to this: related substances is doing more work than the comment implies.

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u/mikkel_correia29 points·2 years ago

That is net peptide content, not purity. Different number, different meaning.

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u/hassan_chowdhury50 points·2 years ago

the related substances line is where the story is

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u/noor_cardoso158 points·2 years ago

The practical rule people converge on is 28 days at fridge temperature after first puncture, and that comes from the preservative, not the peptide.

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u/certified_referenceMOD105 points·2 years ago

Retitled to remove editorialising. Put the evidence in the body.

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u/bastian_moreau86 points·2 years ago

Retitled to remove editorialising.

Disagree on that part. 95.1% and 96.2% on the same vial is normal.

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[deleted]40 points·2 years ago

[deleted]

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u/laila_yilmaz47 points·2 years ago

This is the correct framing. The COA structure matters as much as the number.

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u/wanjiru_varga22 points·2 years ago

the counterion is part of the mass you paid for

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u/laila_yilmaz43 points·2 years ago

Retitled to remove editorialising.

Yes, exactly this, and it is the bit that took me 90 weeks to accept.

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u/first_hundred10 points·2 years ago

Small correction: the trial was 11 weeks, not 28. Does not change your point but people will quote it.

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u/gastric_emptying_g65 points·2 years ago

Dead space in the needle hub holds a small but non-trivial volume. On low-volume draws that can be several units.

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About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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