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c/hplc·submitted 2 years ago by u/ravi_bergstrom

what changed for me between week 90 and week 44

Correctionbranch of 7 comments

Long-ish post, sorry. tl;dr at the bottom. I have been tracking LC-MS against ApoB for 9 weeks because I could not find anyone who had. The correlation is weaker than I expected, which is itself mildly interesting given how confidently people link the two in here. Caveats up front: one person, one lab, one assay, no…

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7 comments, started 2 years ago
u/sofia_petrescu136 points·2 years ago

This is the correct method framing. Column and gradient are load-bearing fields.

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u/cagrilintide_enthusiast100 points·2 years ago

Strongly agree. Largest single impurity tells a different story than total purity.

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u/santiago_villalobos43 points·2 years ago

Slight fix: the number was 97.6, not 98.1. Decimal point, but a fairly consequential one.

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u/egfr_watcher14 points·2 years ago·edited

chromatogram or it did not happen

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u/hamza_weiss54 points·2 years ago

Not to be pedantic but gradient and method development are being used interchangeably and they are not interchangeable in real life.

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u/crosspost_bot_no58 points·2 years ago

Rodent data is rodent data. Dose scaling is not linear and the models tell you what to investigate, not what to expect.

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u/first_hundred30 points·2 years ago

axis labels included, always

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