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c/hplc·submitted 1 year ago by u/rania_okonkwo

three years of purity threads, summarised so you do not have to read them

Labbranch of 4 comments

Right, the method development question again, but with numbers this time. I have 11 data points over 24 weeks. The pattern is consistent enough that I do not think it is chance, and boring enough that nobody is going to screenshot it, which is usually a good sign. The thing I would flag for anyone new: the effect I…

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4 comments, started 1 year ago
u/peak_area_peteanalytical0 points·1 year ago

Did they post the raw trace or just the summary?

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u/cagrilintide_enthusiast1 point·1 year ago

Detection wavelength? purity and that changes at different wavelengths.

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u/aksel_kjaer1 point·1 year ago

Inter-lab variance on this kind of assay is routinely 1–2 points. Different column, different gradient, different integration.

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u/hydration_hank1 point·1 year ago

Strongly agree. Largest single impurity tells a different story than total purity.

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