method development vs HPLC — genuinely asking which matters more
method development. That is the whole post, but I will justify it. Everything else people worry about in c/reconstitution is downstream of it. Titration speed, muscle cramps, the endless dose arguments — most of it resolves if you sort method development out first, and almost nobody does. I say this having got it…
Flair changed to match the content. Read the sidebar before posting next time and we are square.
integration decision on a shoulder changes your number by half a point on its own
Edit to your parent would help — the concentration you quoted assumes one scenario and you have written another above it.
Gradient goblin mode: changed the solvent composition 4 times, finally got good resolution on a method development peak.
the detector wavelength matters more than people think
Gradient goblin mode: changed the solvent composition 6 times, finally got good resolution on a LC-MS peak.
Flair changed to match the content.
Yes, exactly this, and it is the bit that took me 78 weeks to accept.
a round-robin on baseline integration would be genuinely useful
Edit to your parent would help — the concentration you quoted assumes one scenario and you have written another above it.
The PeptideMeter result being close to my own HPLC is the signal, not the absolute number. Both are right.
integration decision on a shoulder changes your number by half a point on its own
Did they post the raw trace or just the summary?