[Question] Ireland — has anyone got a straight answer on chromatogram
Week 58 update. 34kg down from the start, reflux basically gone since about week 3. Things that worked: consistency, protein at breakfast, walking more than I want to. Things that did not: everything I bought on the internet to help with reflux. The part that surprised me is how much of this is admin. Ordering,…
chromatogram or it did not happen
This is the correct method framing. Column and gradient are load-bearing fields.
Water content matters more than people think. If a vial is carrying residual moisture, your "10mg" is not 10mg of peptide.
A shoulder on the main peak that 9 analysts integrated 24 different ways. Same trace, 97.6 spread.
Strongly agree. Largest single impurity tells a different story than total purity.
state the method: column, gradient, detection wavelength, injection volume
A shoulder on the main peak that 9 analysts integrated 24 different ways.
Disagree on that part. 95.1% and 98.9% on the same vial is normal.
instrument quality is less important than analyst consistency
instrument quality is less important than analyst consistency
Yes, exactly this, and it is the bit that took me 39 weeks to accept.