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Single comment threadYou are looking at one branch of integration — 21 things I got wrong before I got it right — 14 comments in the full submission. View in context.
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c/hplc·submitted 2 years ago by u/nnt_nate

integration — 21 things I got wrong before I got it right

Methodbranch of 7 comments

purity. That is the whole post, but I will justify it. Everything else people worry about in c/reconstitution is downstream of it. Titration speed, reflux, the endless dose arguments — most of it resolves if you sort purity out first, and almost nobody does. I say this having got it wrong for 9 months. My eGFR was…

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7 comments, started 2 years ago
u/camila_lindqvist54 points·2 years ago

Titration speed is a side-effect dial far more than an efficacy dial.

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u/freya_baptista32 points·2 years ago·edited

Slight fix: the number was 97.6, not 98.1. Decimal point, but a fairly consequential one.

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u/aksel_kjaer38 points·2 years ago

That is net peptide content, not purity. Different number, different meaning.

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u/elodie_grimaldi30 points·2 years ago

Respectfully this is a sample of one presented as a finding.

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u/phase_two_pete0 points·2 years ago

Strongly agree. Largest single impurity tells a different story than total purity.

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u/nnt_nateOP1 point·2 years ago

Ran the same vial on two columns, different purity, both right, different separation. Method matters more than equipment.

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u/ewan_marchand-1 point·2 years ago

the gradient is doing the separation work, not the column alone

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