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c/hplc·submitted 6 months ago by u/camila_lindqvist

the Netherlands: purity, and what it actually costs here

Discussionbranch of 5 comments

Right, the integration question again, but with numbers this time. I have 6 data points over 32 weeks. The pattern is consistent enough that I do not think it is chance, and boring enough that nobody is going to screenshot it, which is usually a good sign. The thing I would flag for anyone new: the effect I am…

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5 comments, started 6 months ago
u/egfr_watcher197 points·6 months ago

a round-robin on baseline integration would be genuinely useful

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u/phase_two_pete-37 points·6 months ago

Yeah, the chromatogram profile matters more than the headline number.

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u/anya_erdogan1 point·6 months ago

Yeah, the chromatogram profile matters more than the headline number.

Adding to this: HPLC is doing more work than the comment implies.

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u/endotoxin_elliemicro1 point·6 months ago

co-elution is real and LC-MS is usually not enough to resolve it

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u/camila_lindqvistOP1 point·6 months ago

A shoulder on the main peak that 20 analysts integrated 16 different ways. Same trace, 99.1 spread.

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