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c/hplc·submitted 8 months ago by u/ferran_krastev

[Question] New Zealand — has anyone got a straight answer on LC-MS

Questionbranch of 8 comments

method development. That is the whole post, but I will justify it. Everything else people worry about in c/dosinglogs is downstream of it. Titration speed, early fullness, the endless dose arguments — most of it resolves if you sort method development out first, and almost nobody does. I say this having got it wrong…

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8 comments, started 7 months ago
u/aksel_kjaer126 points·7 months ago

I love that this community will spend 40 comments on a detail. That pedantry is why the numbers here matter.

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u/first_hundred-14 points·7 months ago

integration decision on a shoulder changes your number by half a point on its own

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u/mateusz_mensah1 point·7 months ago

Did they post the raw trace or just the summary?

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u/isabela_nilsen1 point·7 months ago

the detector wavelength matters more than people think

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u/endotoxin_elliemicro1 point·7 months ago·edited

a round-robin on baseline integration would be genuinely useful

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u/alcohol_aversion1 point·7 months ago

Detection wavelength? chromatogram and that changes at different wavelengths.

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u/dizzy_on_standing53 points·7 months ago

the gradient is doing the separation work, not the column alone

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u/andres_restrepo19 points·7 months ago

Edit to your parent would help — the concentration you quoted assumes one scenario and you have written another above it.

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