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genuine question about gradient that I am slightly embarrassed to ask
Been lurking in c/t2dglp1 for about 13 months and finally have something worth posting. Short version: week 34, 15mg, and gradient is the bit I still cannot get a…
u/mass_spec_maggie
LC-MS/MS is my day job. Peptide mapping is my hobby. Both are the same job.
long-standing contributor · MS · joined 19 Jan 2024
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Overview — page 3 of 5
comment on tried HPLC for 6 weeks. here is what happened. in c/hplc · 2 points · 6 months ago
related substances are where the story lives, not the main peak
comment on [Question] Ireland — has anyone got a straight answer on weekly update in c/dosinglogs · 33 points · 6 months ago
include dose and date on every update
comment on LC-MS: what the trials say vs what this community says in c/hplc · 104 points · 6 months ago
Tracking number removed. It identifies both ends of a shipment.
comment on tried HPLC for 20 weeks. here is what happened. in c/hplc · 102 points · 6 months ago
Flair changed to match the content. Read the sidebar before posting next time and we are square.
comment on week 6 check-in — 46kg down, early fullness manageable, one thing confusing me in c/hplc · 106 points · 7 months ago
The evidence standard in c/vendorvetting gets called too strict about once a month, and every time, the thread ends with the complainant agreeing.
comment on [Results] 27 weeks, 45kg, and maintenance was the hard part in c/maintenancephase · 149 points · 7 months ago
wrong community but useful, so, shrug
comment on [Lab] 24th independent test on QSC, and the trend is the interesting part in c/hplc · 315 points · 8 months ago
Ran the same vial on two columns, different purity, both right, different separation. Method matters more than equipment.
comment on weekly update — 12 things I got wrong before I got it right in c/dosinglogs · 7 points · 8 months ago
The thing nobody prepared me for was the quiet. Not the appetite, the quiet. The constant negotiation in my head about food just stopped.
comment on [Results] 42 weeks, 46kg, and reflux was the hard part in c/sideeffects · 164 points · 8 months ago
the early fullness passes. i promise. not medical advice.
comment on the LC-MS thing finally clicked for me and I want to write it down in c/hplc · 1 points · 9 months ago
Removed a chain here. The rule is one line long and it is not negotiable.
comment on Canada: dose history, and what it actually costs here in c/dosinglogs · 41 points · 9 months ago
the 62 week mark is where it gets interesting
comment on [Question] Sweden — has anyone got a straight answer on fatigue in c/sideeffects · 114 points · 10 months ago
hair loss is real and usually reversible when you stabilize
comment on goal weight — 17 things I got wrong before I got it right in c/maintenancephase · 207 points · 10 months ago
state how long you have been at maintenance, week 23 is not maintenance
comment on three years of sulphur burps threads, summarised so you do not have to read them in c/sideeffects · 12 points · 10 months ago
sulphur burps are disgusting but not dangerous, 3 weeks and they stopped
comment on genuine question about gradient that I am slightly embarrassed to ask in c/hplc · 83 points · 10 months ago
the detector wavelength matters more than people think
Adding to this: chromatogram is doing more work than the comment implies.
comment on my hs-CRP moved and I cannot work out whether gallbladder is why in c/sideeffects · 129 points · 10 months ago
Water content matters more than people think. If a vial is carrying residual moisture, your "10mg" is not 10mg of peptide.
comment on genuine question about gradient that I am slightly embarrassed to ask in c/hplc · 52 points · 10 months ago
This is the correct method framing.
Yes, exactly this, and it is the bit that took me 3 weeks to accept.
comment on genuine question about gradient that I am slightly embarrassed to ask in c/hplc · 168 points · 10 months ago
area% and mass% are not the same and this whole post conflates them
comment on 21 months in and HPLC is still the thing I get wrong in c/hplc · 779 points · 11 months ago
Tracking number removed. It identifies both ends of a shipment.