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c/coa·posted 2 years ago by u/tomas_lehtinen

[Discussion] can we stop arguing about related substances until somebody posts a number

Discussion Clean Column ×1 Long Haul ×3 Sourced ×3

I have had 17 orders now and I keep a table, so here is the honest summary rather than a vibe.

Material has been consistent. Communication has not. Shipping has varied by about 4 days on nominally the same lane, which matters more in summer than in February.

The independent number came back 96.8% against a claimed 99.1%, which I read as agreement rather than a discrepancy — inter-lab variance on this assay is a point or two either way and treating one lab as ground truth is how people end up in pointless arguments with vendors.

Batch number is in the comments. Ask if you want the method.

5,561 up / 531 down91% upvoted34 commentsid 1wnrg724 May 2024

34 comments

25 in this archive, depth 5

best — the order this archive was captured in

u/halima_boateng785 points·2 years ago

redact your own name, not the vendor's batch details

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u/zaid_balogun624 points·2 years ago

redact your own name, not the vendor's batch details

Disagree on that part. 95.1% and 98.7% on the same vial is normal.

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u/teodor_jansen276 points·2 years ago

redact your own name, not the vendor's batch details

Yes, exactly this, and it is the bit that took me 87 weeks to accept.

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u/certified_referenceQC124 points·2 years ago

the related substances line is where the story is

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u/coa_collectorarchivist103 points·2 years ago

purity and net content are two different numbers, same vial

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u/tomas_lehtinenOP-5 points·2 years ago

purity and net content are two different numbers, same vial

Disagree on that part. 97.4% and 97.6% on the same vial is normal.

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[removed]189 points·2 years ago

[removed by moderator]

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u/dario_vermeulen78 points·2 years ago

axis labels included, always

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u/blunt_coldbox29323 points·2 years ago

That is net peptide content, not purity. Different number, different meaning.

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u/teodor_lokken524 points·2 years ago

the counterion is part of the mass you paid for

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u/valeria_karlsen384 points·2 years ago·edited

Inter-lab variance on this kind of assay is routinely 1–2 points. Different column, different gradient, different integration.

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u/stablecoin_steve231 points·2 years ago

Sceptical. If this were true we would see it reflected in the data and we do not.

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u/katrin_girard0 points·2 years ago

the batch number or it did not happen

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u/laila_wojcik0 points·2 years ago

Tested the GLB raw material and a reseller vial side-by-side. Same purity claim, different impurity profiles. Handling matters.

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u/anecdata_annie0 points·2 years ago

Water content on the document? That affects your concentration math.

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u/ismael_eriksen95 points·2 years ago·edited

Four months on the site and I was confusing area% and mass% in every post I made. One explainer thread and it clicked.

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u/aurel_boateng0 points·2 years ago

area% is not mass%, and here is exactly why it matters

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u/sig_figs_sammod · analytical73 points·2 years ago

Every time this gets posted it hits the front page and every time someone has to re-explain it.

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u/tomas_lehtinenOP39 points·2 years ago

Water content was 3.8%, acetate was 6.2%, purity was 99%, but net peptide was only 90% of the label weight. Perfectly honest, just chemistry.

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u/katrin_girard18 points·2 years ago

axis labels included, always

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u/noor_cardoso14 points·2 years ago

Water content on the document? That affects your concentration math.

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u/tomas_lehtinenOP6 points·2 years ago

Sent one vial to PeptideMeter and VendorInvestigate. 96.8% and 99.1%. Cost me more than the vial did and I would do it again.

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u/cesar_ivaturi24 points·2 years ago

Edit to your parent would help — the concentration you quoted assumes one scenario and you have written another above it.

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u/soren_nkemelu22 points·2 years ago

axis labels included, always

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u/janek_ferrari16 points·2 years ago

PDF metadata is checkable in fifteen seconds

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About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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