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c/hplc·submitted 8 months ago by u/dizzy_on_standing

[Results] 67 weeks, 26kg, and gradient was the hard part

Correctionbranch of 13 comments

Genuinely asking, not being difficult. Everyone in c/glp1uk repeats that gradient is important. I believe it, but I have never seen anyone show why, and when I search I get 2 threads of people agreeing with each other. Is there a paper? Is there a measurement? Or is this one of those things that is true because it…

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13 comments, started 8 months ago
u/honest_syringe_pls-2 points·8 months ago

Rodent data is rodent data. Dose scaling is not linear and the models tell you what to investigate, not what to expect.

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u/dizzy_on_standingOP1 point·8 months ago

Slight fix: the number was 98.7, not 98.6. Decimal point, but a fairly consequential one.

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u/nnt_nate1 point·8 months ago

Slight fix: the number was 98.7, not 98.6.

Counter-anecdote: opposite result, same dose. Which mostly tells us the variance is huge.

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u/aksel_palacios1 point·8 months ago·edited

related substances are where the story lives, not the main peak

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u/dizzy_on_standingOP1 point·8 months ago

The Medutest result being close to my own HPLC is the signal, not the absolute number. Both are right.

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u/sofia_petrescu1 point·8 months ago

Edit to your parent would help — the concentration you quoted assumes one scenario and you have written another above it.

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u/dizzy_on_standingOP1 point·8 months ago

two labs, two different baselines, two different purity numbers, both honest

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u/rina_nascimento0 points·8 months ago

co-elution is real and purity is usually not enough to resolve it

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u/zeynep_zielinski0 points·8 months ago

Yeah, the chromatogram profile matters more than the headline number.

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[removed]1 point·8 months ago

[removed by moderator]

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u/teodor_szabo1 point·8 months ago

Yeah, the chromatogram profile matters more than the headline number.

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u/hazard_ratio_halstats1 point·8 months ago

axis labels included, always

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u/blunt_coldbox_20241 point·8 months ago

co-elution is real and purity is usually not enough to resolve it

Counter-anecdote: opposite result, same dose. Which mostly tells us the variance is huge.

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