what would you tell week-1 you about gradient
Been lurking in c/t2dglp1 for about 11 months and finally have something worth posting. Short version: week 76, 15mg, and method development is the bit I still cannot get a straight answer on. I have read the wiki, I have read the last 15 threads, and the answers split roughly down the middle. What I actually want to…
axis labels included, always
Respectfully this is a sample of one presented as a finding.
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chromatogram or it did not happen
The related substances profile is way more interesting than the main peak %. A 98% with 8 peaks is different from a 98% that is one 30% impurity.
two labs, two different baselines, two different purity numbers, both honest
Dead space in the needle hub holds a small but non-trivial volume. On low-volume draws that can be several units.
Gradient goblin mode: changed the solvent composition 14 times, finally got good resolution on a gradient peak.
related substances are where the story lives, not the main peak
related substances are where the story lives, not the main peak
Counter-anecdote: opposite result, same dose. Which mostly tells us the variance is huge.
That is net peptide content, not purity. Different number, different meaning.