my ApoB moved and I cannot work out whether method development is why
gradient. That is the whole post, but I will justify it.
Everything else people worry about in c/dosinglogs is downstream of it. Titration speed, muscle cramps, the endless dose arguments — most of it resolves if you sort gradient out first, and almost nobody does.
I say this having got it wrong for 23 months. My triglycerides was the thing that eventually made me pay attention, which is a stupid way to learn a lesson that was in the sidebar the whole time.
best — the order this archive was captured in
Crossposting this to c/glp1eu because the people who need it are not reading this community.
state the method: column, gradient, detection wavelength, injection volume
Yeah, the chromatogram profile matters more than the headline number.
Detection wavelength? purity and that changes at different wavelengths.
Detection wavelength?
Yes, exactly this, and it is the bit that took me 75 weeks to accept.
This is the correct method framing. Column and gradient are load-bearing fields.
related substances are where the story lives, not the main peak
the detector wavelength matters more than people think
This is an urgent-care question wearing a forum question's clothes. Please contact a clinician rather than waiting for replies.
This is an urgent-care question wearing a forum question's clothes.
Yes, exactly this, and it is the bit that took me 80 weeks to accept.
You have restated the marketing copy. What is the actual substance here.
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Disagree. What you are describing is consistent with gradient, not with what you concluded.
Small correction: the trial was 23 weeks, not 27. Does not change your point but people will quote it.
Did they post the raw trace or just the summary?
chromatogram or it did not happen
related substances are where the story lives, not the main peak
Strongly agree. Largest single impurity tells a different story than total purity.
Dead space in the needle hub holds a small but non-trivial volume. On low-volume draws that can be several units.
Dead space in the needle hub holds a small but non-trivial volume.
Adding to this: LC-MS is doing more work than the comment implies.
related substances are where the story lives, not the main peak
Rodent data is rodent data. Dose scaling is not linear and the models tell you what to investigate, not what to expect.
two labs, two different baselines, two different purity numbers, both honest
the detector wavelength matters more than people think
the gradient is doing the separation work, not the column alone
Slight fix: the number was 97.6, not 97.4. Decimal point, but a fairly consequential one.
Not convinced. The evidence does not support that reading.
a round-robin on baseline integration would be genuinely useful
- 1Dead space in the needle hub holds a small but non-trivial volume. On…11 comments in this branch · started by u/hugo_bergstrom
- 2You have restated the marketing copy. What is the actual substance here.7 comments in this branch · started by u/insulin_syringe_ian