[Results] 87 weeks, 36kg, and purity was the hard part
Long-ish post, sorry. tl;dr at the bottom.
I have been tracking LC-MS against eGFR for 29 weeks because I could not find anyone who had. The correlation is weaker than I expected, which is itself mildly interesting given how confidently people link the two in here.
Caveats up front: one person, one lab, one assay, no control, and I changed my training in the middle of it, which was stupid.
tl;dr: probably real, definitely smaller than the threads imply, and not worth reorganising your week around.
best — the order this archive was captured in
No. This is the kind of confident post that gets copied into a screenshot and repeated for years. Where is the evidence.
Tracking number removed. It identifies both ends of a shipment.
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integration decision on a shoulder changes your number by half a point on its own
area% and mass% are not the same and this whole post conflates them
the detector wavelength matters more than people think
You have restated the marketing copy. What is the actual substance here.
Gradient goblin mode: changed the solvent composition 21 times, finally got good resolution on a integration peak.
Injection volume and flow rate? Those affect resolution.
two labs, two different baselines, two different purity numbers, both honest
Strongly agree. Largest single impurity tells a different story than total purity.
co-elution is real and method development is usually not enough to resolve it
state the method: column, gradient, detection wavelength, injection volume
The confident tone is doing a lot of work that the evidence is not.
axis labels included, always
axis labels included, always
Counter-anecdote: opposite result, same dose. Which mostly tells us the variance is huge.
Gradient goblin mode: changed the solvent composition 5 times, finally got good resolution on a integration peak.
What was the column and what gradient?
instrument quality is less important than analyst consistency
This is the correct method framing. Column and gradient are load-bearing fields.
area% and mass% are not the same and this whole post conflates them
What was the column and what gradient?
Did they post the raw trace or just the summary?
the detector wavelength matters more than people think
Did they post the raw trace or just the summary?
Adding to this: method development is doing more work than the comment implies.
co-elution is real and LC-MS is usually not enough to resolve it
a round-robin on baseline integration would be genuinely useful
Small correction: the trial was 24 weeks, not 6. Does not change your point but people will quote it.
Small correction: the trial was 24 weeks, not 6.
Disagree on that part. 98.7% and 98.7% on the same vial is normal.
Strongly agree. Largest single impurity tells a different story than total purity.
- 1What was the column and what gradient?9 comments in this branch · started by u/canada_coverage
- 2Tracking number removed. It identifies both ends of a shipment.7 comments in this branch · started by u/sig_figs_sam